kinase domains ephb1  (Addgene inc)

Journal: Molecular Vision

Article Title: EphB1 causes retinal damage through inflammatory pathways in the retina and retinal Müller cells

doi:

Figure Lengend Snippet: EphB1 protein levels are increased in the retinas of diabetic patients and diabetic mice. Western blotting was done for EphB1 in whole retinal lysates from (A) control and diabetic patients and (B) control and diabetic mice. All diabetic patients had had the disease for over 10 years. *p<0.05 versus control. n=7 for both panels. Data are presented as mean ± standard error of mean (SEM).

Article Snippet: Some cells in high glucose were treated with siRNA to EphB1 (Origene) or scrambled siRNA transfected into the cells using RNAiMax.

Techniques: Western Blot, Control

Journal: Molecular Vision

Article Title: EphB1 causes retinal damage through inflammatory pathways in the retina and retinal Müller cells

doi:

Figure Lengend Snippet: EphB1 is increased in rMC-1 Müller cells. Panel A shows the localization of EphB1 in rMC-1 cells in culture in normal glucose (top) and high glucose (bottom). Cells are stained with green for EphB1 and DAPI for nuclear localization. Panel B is western blot data from rMC-1 cells grown in normal (5 mM) or high (25 mM) glucose. *p<0.05 versus normal glucose (NG), n=5 for the western blot. Data are presented as mean ± SEM. Scale bar is 25 µm.

Article Snippet: Some cells in high glucose were treated with siRNA to EphB1 (Origene) or scrambled siRNA transfected into the cells using RNAiMax.

Techniques: Staining, Western Blot

Journal: Molecular Vision

Article Title: EphB1 causes retinal damage through inflammatory pathways in the retina and retinal Müller cells

doi:

Figure Lengend Snippet: EphB1 siRNA reduced inflammatory mediators in rMC-1 cells. rMC-1 cells were grown in normal (5 mM) or high (25 mM) glucose. Some cells in high glucose were treated with EphB1 siRNA or scrambled siRNA. Western blotting was performed for (A) EphB1, (B) TNFα, and (C) NLRP3. An ELISA was done for IL-1β in panel D. *p<0.05 versus NG, #p<0.05 versus high glucose (HG). n=5. Data are presented as mean ± SEM.

Article Snippet: Some cells in high glucose were treated with siRNA to EphB1 (Origene) or scrambled siRNA transfected into the cells using RNAiMax.

Techniques: Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Molecular Vision

Article Title: EphB1 causes retinal damage through inflammatory pathways in the retina and retinal Müller cells

doi:

Figure Lengend Snippet: Ephrin B1 increased inflammatory mediators in rMC-1 cells. rMC-1 cells were grown in normal (5 mM) or high (25 mM) glucose. Some cells in each glucose condition were treated with ephrin B1-Fc. Western blotting was performed for (A) HMGB1 and (B) NLRP3. An ELISA was done for IL-1β. *p<0.05 versus NG, #p<0.05 versus HG. n=5. Data are presented as mean ± SEM.

Article Snippet: Some cells in high glucose were treated with siRNA to EphB1 (Origene) or scrambled siRNA transfected into the cells using RNAiMax.

Techniques: Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Molecular Vision

Article Title: EphB1 causes retinal damage through inflammatory pathways in the retina and retinal Müller cells

doi:

Figure Lengend Snippet: EphB1 can be expressed by AAV in Müller cells. To express EphB1 in retinal Müller cells in mice, mice were injected equal amounts of either the EphB1 overexpression AAV or control AAV. The left panel (green) shows HA staining (as a control), the middle panel is EphB1 (red), and the merged image is on the right with yellow staining demarcated by arrows showing co-localization. Five mice in each group were used for the staining experiments. Scale bar is 50 nm.

Article Snippet: Some cells in high glucose were treated with siRNA to EphB1 (Origene) or scrambled siRNA transfected into the cells using RNAiMax.

Techniques: Injection, Over Expression, Control, Staining

Journal: Molecular Vision

Article Title: EphB1 causes retinal damage through inflammatory pathways in the retina and retinal Müller cells

doi:

Figure Lengend Snippet: Ischemia/reperfusion damage was worsened by overexpression of EphB1 by AAV. The top panels show retinal thickness and cell loss in the ganglion cell layer. Quantification of cell loss and retinal thickness is shown in graphs below the images. *p<0.05 versus AAV control, #p<0.05 versus AAV control I/R. n=5 for all groups. Data are mean ± SEM.

Article Snippet: Some cells in high glucose were treated with siRNA to EphB1 (Origene) or scrambled siRNA transfected into the cells using RNAiMax.

Techniques: Over Expression, Control